A Secret Weapon For flow cytometers
A Secret Weapon For flow cytometers
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Knowledge-enriched characterization of mobile populations. Morphological facts from photos can add on the richness of flow cytometry details. For instance, the figure reveals an if not common apoptosis assay applying Annexin V and PI, introducing cell imaging to characterize cells in Each individual population to reveal morphologically distinct options. These insights could not have already been acquired from multiplex staining by yourself.
As samples are obtained to the Attune CytPix Flow Cytometer, the substantial-pace brightfield camera captures and stores photos of detected gatherings at a charge nearly 6,000 pictures for each 2nd, depending upon the flow level and graphic dimensions. For bigger overall flexibility, Attune Cytometric Software permits you to modify the image seize frequency as essential and choose a particular gate for graphic seize, if expected.
Incorporate acoustic focusing technologies with high-parameter Investigation for adaptable spectral unmixing and standard workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating that has a superior-velocity brightfield digicam for simultaneous higher throughput flow cytometry and superior resolution brightfield imaging.
"A picture just isn't really truly worth one thousand dots, nevertheless it actually really helps to see photos. It is great to see visuals that correlate with staining. By way of example, AnnexinV-good cells which can be morphologically distinct than balanced cells, or CD14+ monocytes which are larger sized plus more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it is actually illuminating to see the quantity of doublets slip by means of standard singlet gates, and great to confirm the status of unpredicted double-constructive occasions in my Evaluation."
Optimizing gating approach. Even robust manual singlet gating is mistake-inclined and continues to be a subjective choice position in almost all flow cytometry assays. Imaging can be utilized to substantiate and regulate gates to incorporate only single cells of fascination.
Our scientists will design and style panels for you personally as repeatedly you would like—cost-free, no product or service purchase required.
The Attune NxT cytometer is the responsible workhorse instrument your lab can depend upon for reliable and reliable functionality. The Attune CytPix product contains a superior-speed brightfield digicam and AI-driven graphic Assessment software program, with modern upgrades which include device Understanding applications and an intuitive person interface, enabling enhanced facts precision, precision, and quicker insights into sample biology.
Probably most significantly, these functions contain cells of Plainly diverse phenotypes which may lead to incorrect conclusions with regards to double constructive situations (especially in uncommon populations).
Seem waves allow exact cell alignment, resulting in Remarkable details quality and ~10X more quickly sample flow prices.
We Beforehand shown the strength of imaging Car or truck-T/Ramos mobile interactions. Allow’s evaluate just the populace of biggest fascination, the double positive events, To find out more. We could now use prolonged morphological parameters (below, circularity here and skewness of depth) to even more examine the attributes of these populations and refine gating on these situations, escalating details robustness.
Built-in dimensionality reduction equipment greater resolution into populations. Fluorescently gated populations again-gated on to UMAP plot to verify envisioned population resolution in lysed complete human blood. Consumer can watch gatherings from Just about every cluster for Visible confirmation of mobile phenotypes.
Versatile payment approach from Agilent Monetary Alternatives let you get the critical instrumentation you would like for instant task, or to expand your lab.
Gating on these prolonged parameters enables you to swiftly and properly discover populations of interest and ensure gating system with little or no handbook review. The Attune software delivers use of point out-of-the-art machine Discovering Investigation resources within just an expertly made consumer interface, rendering it exceptionally convenient to use.
Since the mobile line had previously been analyzed within the Attune CytPix Flow Cytometer, the scientists went again to the images and were being in the position to doc the microbial an infection at the very least 5 times before.
The Attune CytPix Flow Cytometer lets customers to visualize samples during acquisition, verify the precision of gating techniques, and help outcomes with reputable documentation for publication needs.
What gave the impression to be a homogeneous populace is definitely far more diverse—an Perception that should be considered when interpreting effects.